| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88 Mu g/mL |
| Detection Limit: |
3.13~200 Mu g/mL |
| Short Description: |
This Alb Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Alb |
| Gene ID: |
24186 |
| Uniprot ID: |
ALBU_RAT |
| Specificity: |
This kit recognizes Rat ALB in samples. No significant cross-reactivity or interference between Rat ALB and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. |
| Function | Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood. The rank order of affinity is zinc > calcium > magnesium. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin. |
| Protein Name | Albumin |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-159418Reactome: -RNO-189451Reactome: -RNO-189483Reactome: -RNO-2168880Reactome: -RNO-381426Reactome: -RNO-8957275Reactome: -RNO-8964058Reactome: -RNO-9707564Reactome: -RNO-9749641Reactome: -RNO-9757110Reactome: -RNO-9793528 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Albumin ELISA kitAlb ELISA kit |
| Specificity | This kit recognizes Rat ALB in samples. No significant cross-reactivity or interference between Rat ALB and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-96 | 91 |
| EDTA plasma (n=8) | 86-100 | 91 |
| Cell culture media (n=8) | 95-107 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (μg/mL) | 9.90 | 25.50 | 76.00 | 9.80 | 23.30 | 78.50 |
| Standard deviation | 0.50 | 1.20 | 3.60 | 0.50 | 1.20 | 2.70 |
| CV (%) | 5.05 | 4.71 | 4.74 | 5.10 | 5.15 | 3.44 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
