• Rat Acta2 (Actin, aortic smooth muscle) Sandwich ELISA Kit (STJE0006393)
  • Rat Acta2 (Actin, aortic smooth muscle) Sandwich ELISA Kit (STJE0006393)

Rat Acta2 (Actin, aortic smooth muscle) Sandwich ELISA Kit (STJE0006393)

SKU:
STJE0006393

Current Stock:
Applications: ELISA
Reactivity: Rat
Note: FOR RESEARCH USE ONLY (RUO).
Sensitivity: 1.88ng/mL
Detection Limit: 3.13~200ng/mL
Short Description: This Acta2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8 °C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Acta2
Gene ID: 81633
Uniprot ID: ACTA_RAT
Specificity: This kit recognizes Rat ACT Alpha 2 in samples. No significant cross-reactivity or interference between Rat ACT Alpha 2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization. Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. Methylated at His-75 by SETD3.
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Protein Name Actin - Aortic Smooth Muscle
Alpha-Actin-2 Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form
Database Links Reactome: R-RNO-445355
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative ELISA Names Actin - Aortic Smooth Muscle ELISA kit
Alpha-Actin-2 Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form ELISA kit
Acta2 ELISA kit
Actsa ELISA kit
Actvs ELISA kit
Specificity This kit recognizes Rat ACTα2 in samples. No significant cross-reactivity or interference between Rat ACTα2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 95
EDTA plasma (n=8) 87-102 94
Cell culture media (n=8) 84-98 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 9.55 22.09 81.47 9.42 22.25 89.17
Standard deviation 0.52 1.09 4.47 0.64 1.04 4.53
CV (%) 5.45 4.93 5.49 6.79 4.67 5.08
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance