| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Profilin Positive Control for STJ506120 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | PFN1 |
| Gene ID: | 5216 |
| Uniprot ID: | PROF1_HUMAN |
| Specificity: | This is positive control is recommended for use in combination with Profilin antibody STJ506120. |
| Tissue Specificity | Expressed in epididymis (at protein level). |
| Post Translational Modifications | Phosphorylation at Ser-138 reduces its affinity for G-actin and blocks its interaction with HTT, reducing its ability to inhibit androgen receptor (AR) and HTT aggregation. |
| Function | Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR. |
| Peptide Name | Profilin-1 Epididymis Tissue Protein Li 184a Profilin I |
| Database Links | Reactome: R-HSA-114608 Reactome: R-HSA-376176 Reactome: R-HSA-4086400 Reactome: R-HSA-5663220 |
| Cellular Localisation | Cytoplasm Cytoskeleton |
| Alternative Peptide Names | Profilin-1 protein Epididymis Tissue Protein Li 184a protein Profilin I protein PFN1 protein |
Information sourced from Uniprot.org