Loading Control Antibody | St John's Laboratory

Immunofluorescence analysis of rat testis cells using Acetyl-alpha Tubulin-K40 Rabbit polyclonal antibody (STJ11105006) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-Acetyl-TUBA1A-K40 antibody (STJ11105006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Immunohistochemistry with Anti-ACTB Antibody (STJ500039) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Right slide is negative control.

Anti-ACTB antibody (1-50) (STJ500039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

€534.38

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Anti-ACTB antibody (1-50) {Biotin} (STJ500043)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

€660.63

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Anti-ACTB antibody (1-50) {FITC} (STJ500044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

€660.63

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Western blot analysis of customer's lysis using Actin Beta antibody. Antibody was diluted at 1:2000

Anti-ACTB antibody (1-80 aa) (STJ91464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Chicken/Globefish/Cow/Hamster/Pig/Sheep/Cat/Pig/Dog/Sheep

€60.00 - €315.00

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Anti-beta-Actin antibody at 1:500 dilution; lysates at 100 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-ACTB antibody (100aa N-Term) (STJ140081)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

€126.88 - €493.13

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Anti-ACTB antibody (C-Term) [M202] (STJA0005244)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Chicken/Human/Mouse/Rat

€399.38

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Anti-ACTB antibody (N-Term) (STJA0005241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IHC/IP

Reactivity :

Chicken/Human/Mouse/Rat

€399.38

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Immunofluorescence analysis of Hela cell. 1, Cyclin A Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-actin monoclonal antibody (5B7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-ACTB antibody [5B7] (STJ96930)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect/Pig/Lenok

€60.00 - €315.00

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Anti-ACTB antibody [5B7] {AbFluor™ 350} (STJA0006194)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {AbFluor™ 405} (STJA0006195)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {AbFluor™ 488} (STJA0006196)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {AbFluor™ 555} (STJA0006197)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {AbFluor™ 594} (STJA0006198)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {AbFluor™ 647} (STJA0006199)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {AbFluor™ 680} (STJA0006200)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {Biotin} (STJA0006201)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {Cy3} (STJA0006202)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {Cy5} (STJA0006203)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {FITC} (STJA0006204)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {HRP} (STJA0006102)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [M008] (STJA0005243)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

€489.38

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Western blot analysis of various lysates using Beta-Actin Rabbit mAb (STJ11100960) at 1:50000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-ACTB antibody [S0MR] (STJ11100960)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Chicken/Zebrafish/Pig

€169.38 - €311.88

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Western blot analysis of various lysates using Beta-Actin Rabbit mAb (High Dilution) (STJ113532) at 1:100000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ACTB antibody [S2MR] (STJ113532)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Chicken/Zebrafish/Pig

€216.88 - €406.88

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Western blot analysis of various lysates using HRP-conjugated Beta-Actin Rabbit mAb (STJ116402) at 1:50000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-ACTB antibody [S2MR] (STJ116402)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Chicken/Zebrafish/Pig

€120.63 - €490.63

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Western blot analysis of various lysates using HRP-conjugated Beta-Actin Mouse mAb (STJ11105764) at 1:5000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-ACTB antibody [S5764MM] {HRP} (STJ11105764)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€169.38 - €311.88

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Western blot analysis of various cells using Actin-Kappa Polyclonal Antibody

Anti-ACTBL2 antibody (81-130 aa) (STJ91470)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-Actin antibody (C-Term) (STJ13100007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

€414.38

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Anti-Actin antibody (C-Term) (STJ13100011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

€414.38

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Actin, sarcomeric muscle (ABT-SCA)) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: mouse heart Lane 2: mouse skeletal musclePredicted band size: 42kDaObserved band size: 42kDa

Anti-Actin, sarcomeric muscle antibody (2-50 aa) [ABT-SCA] (STJ197005)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

€217.50 - €315.00

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Western blot analysis of lysates from Tobacco, using Actin (plant specific) Rabbit pAb (STJ11107558) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-Actin-Plant Specific antibody (STJ11107558)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Various Plants

€169.38 - €311.88

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Western blot analysis of lysates from Arabidopsis thaliana tissue using Actin (plant specific) Mouse mAb (STJ11107554). <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-Actin-Plant Specific antibody [SMM] (STJ11107554)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Various Plants

€169.38 - €311.88

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Western blot analysis of a-Tubulin at dilution of 1:2000

Anti-alpha Tubulin antibody [SM5232] (STJA0005232)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

€213.13 - €291.88

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Western blot analysis of various lysates using Alpha-Tubulin Mouse mAb (STJ11107556) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Alpha-Tubulin antibody [SMM] (STJ11107556)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€80.63 - €375.63

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Anti-ATAT1 antibody (STJA0009559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of Beta-III-Tubulin at dilution of 1:1000

Anti-beta III Tubulin antibody [SM0434] (STJA0010434)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

€213.13 - €291.88

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Western blot analysis of various lysates, using Beta III-Tubulin Rabbit pAb (STJ11106048) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Beta III-Tubulin antibody (40-260 aa) (STJ11106048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various lysates using Beta III-Tubulin Mouse mAb (STJ11106837) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Beta III-Tubulin antibody (40-260 aa) [SMM] (STJ11106837)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Anti-beta Tubulin antibody (C-Term) (STJ13100428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human/Xenopus/Zebrafish

€414.38

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Western blot analysis of TUBB5 at dilution of 1:1000

Anti-beta Tubulin antibody [SM5085] (STJA0005085)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

€115.63

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Western blot analysis of various lysates using Beta-Actin Rabbit pAb (STJ11103684) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Beta-Actin antibody (1-375) (STJ11103684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€169.38 - €311.88

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Western blot analysis of lysates from HeLa cells, using Beta-Actin Mouse mAb (STJ11103683) at 1:2000-1:640000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Beta-Actin antibody [S3MR] (STJ11103683)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€169.38 - €311.88

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Western blot analysis of various lysates using Beta-Tubulin Mouse mAb (STJ11107557) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Beta-Tubulin antibody [SMM] (STJ11107557)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Hamster/Monkey/Goat/C.reinhardtii

€200.63 - €375.63

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Western blot analysis of various lysates using HRP-conjugated Beta-Tubulin Mouse mAb (STJ11107559) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Beta-Tubulin antibody [SMM] {HRP} (STJ11107559)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€216.88 - €406.88

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Western blot analysis of various lysates, using Gamma-Tubulin Rabbit mAb (STJ11106968) at1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-Gamma-Tubulin antibody [SRM] (STJ11106968)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Western blot analysis of various lysates using GAPDH Rabbit pAb (STJ11103681) at 1:30000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-GAPDH antibody (1-335) (STJ11103681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP

Reactivity :

Human/Mouse/Rat

€169.38 - €311.88

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Western blot analysis of various lysates using GAPDH Rabbit pAb (STJ11103691) at 1:10000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-GAPDH antibody (1-335) (STJ11103691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP

Reactivity :

Human/Mouse/Rat

€169.38 - €311.88

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Western blot analysis of various lysates using GAPDH Mouse mAb (STJ11103682) at 1:10000 dilution incubated overnight at 4°C. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-GAPDH antibody (1-335) [S2MR] (STJ11103682)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€169.38 - €311.88

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Loading Control Antibodies