Western Blot Antibodies | St John's Laboratory

Human peripheral blood lymphocytes stained with CD3E+CD3G Rabbit monoclonal antibody (STJ11103942) (1 Mu g/mL) (orange). Secondary antibody only staining (Blue) and unstained sample (red) were used as a control.

Anti-CD3E+CD3G antibody (1-207) [S3942RM] (STJ11103942)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human

£129.50 - £574.50

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STJ73089 (0. 1µg/ml) staining of Jurkat (A) and Molt4 (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD3E-Cytoplasmic antibody (Internal) (STJ73089)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Rat/Pig

£312.50

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Anti-CD3G antibody (131-181) (STJ500451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£427.50

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Anti-CD3G antibody (131-181) {Biotin} (STJ500466)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£528.50

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Anti-CD3G antibody (131-181) {FITC} (STJ500467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£528.50

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Western blot analysis of various lysates, using CD3G antibody (STJ11103929) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-CD3G antibody (23-116) (STJ11103929)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Flow cytometry: 1X10^6 Raji cells (negative control, left) and Jurkat cells (right) were intracellularly-stained with CD3G Rabbit monoclonal antibody (STJ11103551, 2. 5 Mu g/mL orange line) or ABflo® 488 Rabbit IgG isotype control ( 2. 5 Mu g/mL, blue line). Non-fluorescently stained cells were used as blank control (red line).

Anti-CD3G antibody (83-182) [S1MR] (STJ11103551)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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STJ73095 (0. 3µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD3G antibody (Internal) (STJ73095)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD3G antibody (STJ192944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD4 (PTR2055) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: JurkatPredicted band size: 51kDaObserved band size: 51kDa

Anti-CD4 antibody (1-100) [PTR2055] (STJA0006231)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£48.00 - £252.00

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Immunofluorescence analysis of Jurkat cells using CD4 Rabbit polyclonal antibody (STJ114878) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CD4 antibody (26-200) (STJ114878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded mouse spleen using CD4 Rabbit polyclonal antibody (STJ114879) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD4 antibody (26-200) (STJ114879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Jurkat cells using CD4 Rabbit polyclonal antibody (STJ26171) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD4 antibody (26-317) (STJ26171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of THP-1 cells using CD4 Rabbit polyclonal antibody (STJ11105070) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD4 antibody (27-394 aa) (STJ11105070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot with Anti-CD4 Antibody (STJ500468) with peptide on at 1:500 dilution in buffer.

Anti-CD4 antibody (270-320) (STJ500468)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse

£427.50

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Anti-CD4 antibody (270-320) {Biotin} (STJ500470)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse

£528.50

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Anti-CD4 antibody (270-320) {FITC} (STJ500471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse

£528.50

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Western blot analysis of various cells using CD4 Polyclonal Antibody diluted at 1：500

Anti-CD4 antibody (401-450 aa) (STJ92127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry of mouse spleen with Anti-CD4 primary antibody (STJ500469) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CD4 antibody (437-458) (STJ500469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-CD4 antibody (437-458) {Biotin} (STJ500472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-CD4 antibody (437-458) {FITC} (STJ500473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Endogenous CD4 detected at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-CD4 antibody (50-235aa ECD) (STJ140023)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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STJ73121 (1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD4 antibody (Internal) (STJ73121)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/FC

Reactivity :

Human

£312.50

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Anti-Cd4 antibody [GK1.5] (STJA0001615)

Clonality :

Monoclonal

Host :

Rat

Applications :

Depletion/Block/FC/WB/IHC/IP

Reactivity :

Mouse

£93.50 - £188.50

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Anti-Cd4 antibody [GK1.5] (STJA0001616)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/WB/IHC/IP

Reactivity :

Mouse

£93.50 - £114.50

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Anti-CD4 antibody [MEM-241] (STJ16100165)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

£405.50

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Anti-CD4 antibody [SAA2002] (STJA0020549)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/WB

Reactivity :

Human

£341.50 - £1,447.50

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Anti-CD4 antibody [SRM] (STJ11107140)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Anti-CD4 antibody [SRM] (STJ11107141)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Immunofluorescence analysis of mouse spleen using CD40 Rabbit polyclonal antibody (STJ23000) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD40 antibody (198-277) (STJ23000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates, using CD40 antibody (STJ11104929) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD40 antibody (21-193 aa) [S4929RM] (STJ11104929)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various cells using CD40 Polyclonal Antibody

Anti-CD40 antibody (228-277 aa) (STJ92128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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Immunohistochemistry analysis of CD40 in paraffin-embedded mouse spleen using CD40 Rabbit polyclonal antibody (STJ11101171) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CD40 antibody (25-104) (STJ11101171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CD40/TNFRSF5 antibody [SAA2006] (STJA0020553)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/WB

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of extracts of various cell lines, using CD40L antibody (STJ23001) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-CD40LG antibody (113-261) (STJ23001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of PC-12 cells using CD40L antibody (STJ114971) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CD40LG antibody (50-150) (STJ114971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CD40LG antibody (STJA0010240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-CD42b/GP1BA antibody [5G6] (STJA0027300)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

£203.50 - £294.50

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Anti-CD42b/GP1BA antibody [5G6] {APC} (STJA0029047)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

£246.50 - £363.50

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Anti-CD42b/GP1BA antibody [5G6] {FITC} (STJA0028081)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

£203.50 - £294.50

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Anti-CD42b/GP1BA antibody [5G6] {PerCP} (STJA0030013)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

£231.50 - £339.50

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Anti-CD42b/GP1BA antibody [5G6] {PE} (STJA0030979)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

£246.50 - £363.50

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Western blot analysis of lysates from HEL cells using CD42d Rabbit pAb (STJ11107110) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-CD42d antibody (17-477 aa) (STJ11107110)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-CD43 antibody [84-3C1] (STJ16101106)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human

£287.50 - £1,324.50

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Anti-CD43 antibody [DF-1] (STJ16101856)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/WB

Reactivity :

Human

£682.50

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Anti-CD43 antibody [MEM-59] (STJ16100061)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB/IHC-P

Reactivity :

Human

£431.50

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Anti-CD43b antibody [Bra7G] (STJ16101869)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human

£307.50 - £1,449.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD44 antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 81 kDa

Anti-CD44 antibody (100-200 aa) [ABT147] (STJ197122)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human

£174.00 - £252.00

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