| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.75ng/mL |
| Detection Limit: |
1.25~80ng/mL |
| Short Description: |
This Vwf Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Vwf |
| Gene ID: |
22371 |
| Uniprot ID: |
VWF_MOUSE |
| Specificity: |
This kit recognizes Mouse VWF in samples. No significant cross-reactivity or interference between Mouse VWF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | All cysteine residues are involved in intrachain or interchain disulfide bonds. N- and O-glycosylated. |
| Function | Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. |
| Protein Name | Von Willebrand FactorVwf Cleaved Into - Von Willebrand Antigen 2Von Willebrand Antigen Ii |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-140837Reactome: -MMU-216083Reactome: -MMU-354192Reactome: -MMU-354194Reactome: -MMU-372708Reactome: -MMU-430116Reactome: -MMU-5674135Reactome: -MMU-75892Reactome: -MMU-76009 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixLocalized To Storage Granules |
| Alternative ELISA Names | Von Willebrand Factor ELISA kitVwf Cleaved Into - Von Willebrand Antigen 2 ELISA kitVon Willebrand Antigen Ii ELISA kitVwf ELISA kit |
| Specificity | This kit recognizes Mouse VWF in samples. No significant cross-reactivity or interference between Mouse VWF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-101 | 92 |
| EDTA plasma (n=8) | 93-104 | 99 |
| Cell culture media (n=8) | 91-102 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 4.33 | 12.59 | 29.76 | 4.41 | 13.12 | 27.83 |
| Standard deviation | 0.23 | 0.75 | 1.48 | 0.30 | 0.64 | 1.45 |
| CV (%) | 5.31 | 5.96 | 4.97 | 6.80 | 4.88 | 5.21 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
