| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
3.21pg/mL |
| Detection Limit: |
7.81-500pg/mL |
| Short Description: |
This VEGF-A Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Vegfa |
| Gene ID: |
22339 |
| Uniprot ID: |
VEGFA_MOUSE |
| Specificity: |
This kit recognizes Mouse VEGF-A in samples. No significant cross-reactivity or interference between Mouse VEGF-A and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form. |
| Function | N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. |
| Protein Name | Vascular Endothelial Growth Factor A - Long FormL-VegfVascular Permeability FactorVpf Cleaved Into - N-Vegf - Vegfa |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-194313Reactome: -MMU-195399Reactome: -MMU-4420097Reactome: -MMU-5218921 |
| Cellular Localisation | N-Vegf: CytoplasmNucleusCytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic ConditionsVegfa: SecretedIsoform L-Vegf-2: Endoplasmic ReticulumGolgi ApparatusSecretedExtracellular SpaceExtracellular MatrixIsoform Vegf-1: SecretedIsoform Vegf-2: SecretedIsoform Vegf-3: Cell MembranePeripheral Membrane ProteinRemains Cell-Surface Associated Unless Released By Heparin |
| Alternative ELISA Names | Vascular Endothelial Growth Factor A - Long Form ELISA kitL-Vegf ELISA kitVascular Permeability Factor ELISA kitVpf Cleaved Into - N-Vegf - Vegfa ELISA kitVegfa ELISA kitVegf ELISA kit |
| Specificity | This kit recognizes Mouse VEGF-A in samples. No significant cross-reactivity or interference between Mouse VEGF-A and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-105 | 102 |
| EDTA plasma (n=8) | 93-100 | 96 |
| Cell culture media (n=8) | 87-98 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 22.41 | 58.49 | 215.21 | 20.44 | 50.95 | 220.39 |
| Standard deviation | 1.32 | 2.81 | 10.16 | 1.03 | 2.39 | 10.01 |
| CV (%) | 5.91 | 4.81 | 4.72 | 5.06 | 4.69 | 4.54 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
