| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.28ng/mL |
| Detection Limit: |
0.781-50ng/mL |
| Short Description: |
This TFR Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Tfrc |
| Gene ID: |
22042 |
| Uniprot ID: |
TFR1_MOUSE |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Stearoylated by ZDHHC6 which inhibits TFRC-mediated activation of the JNK pathway and promotes mitochondrial fragmentation. Stearoylation does not affect iron uptake. N- and O-glycosylated, phosphorylated and palmitoylated. |
| Function | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Upon stimulation, positively regulates T and B cell proliferation through iron uptake. Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway. When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion. When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2. |
| Protein Name | Transferrin Receptor Protein 1TrTfrTfr1TrfrCd Antigen Cd71 |
| Database Links | Reactome: R-MMU-432722Reactome: -MMU-8856825Reactome: -MMU-8856828Reactome: -MMU-8980692Reactome: -MMU-9013026Reactome: -MMU-9013106Reactome: -MMU-9013149Reactome: -MMU-9013404Reactome: -MMU-9013406Reactome: -MMU-9013407Reactome: -MMU-9013408Reactome: -MMU-9013423Reactome: -MMU-917977Reactome: -MMU-9696270Reactome: -MMU-9696273 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinMelanosome |
| Alternative ELISA Names | Transferrin Receptor Protein 1 ELISA kitTr ELISA kitTfr ELISA kitTfr1 ELISA kitTrfr ELISA kitCd Antigen Cd71 ELISA kitTfrc ELISA kitTrfr ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
