| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This mouse SELL kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Sell |
| Gene ID: |
20343 |
| Uniprot ID: |
LYAM1_MOUSE |
| Specificity: |
This kit recognizes Mouse SELL in samples. No significant cross-reactivity or interference between Mouse SELL and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Predominantly expressed in lymphoid tissue. |
| Post Translational Modifications | N-glycosylated. |
| Function | Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. |
| Protein Name | L-SelectinCd62 Antigen-Like Family Member LLeukocyte Adhesion Molecule 1Lam-1Leukocyte-Endothelial Cell Adhesion Molecule 1Lecam1Lymph Node Homing ReceptorLymphocyte Antigen 22Ly-22Lymphocyte Surface Mel-14 AntigenCd Antigen Cd62l |
| Database Links | Reactome: R-MMU-198933Reactome: -MMU-202733Reactome: -MMU-6798695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative CLIA Names | L-Selectin CLIA kitCd62 Antigen-Like Family Member L CLIA kitLeukocyte Adhesion Molecule 1 CLIA kitLam-1 CLIA kitLeukocyte-Endothelial Cell Adhesion Molecule 1 CLIA kitLecam1 CLIA kitLymph Node Homing Receptor CLIA kitLymphocyte Antigen 22 CLIA kitLy-22 CLIA kitLymphocyte Surface Mel-14 Antigen CLIA kitCd Antigen Cd62l CLIA kitSell CLIA kitLnhr CLIA kitLy-22 CLIA kitLy22 CLIA kit |
| Specificity | This kit recognizes Mouse SELL in samples. No significant cross-reactivity or interference between Mouse SELL and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-108 | 101 |
| EDTA plasma(n=8) | 93-106 | 99 |
| Cell culture media(n=8) | 91-105 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 102.10 | 183.42 | 901.12 | 109.05 | 200.90 | 960.74 |
| Standard deviation | 11.03 | 14.55 | 67.67 | 9.87 | 23.36 | 108.95 |
| CV (%) | 10.80 | 7.93 | 7.51 | 9.05 | 11.63 | 11.34 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
