| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
32.15 pg/mL |
| Detection Limit: |
78.13-5000 pg/mL |
| Short Description: |
This SDF-1/CXCL12 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Cxcl12 |
| Gene ID: |
20315 |
| Uniprot ID: |
SDF1_MOUSE |
| Specificity: |
This kit recognizes Mouse SDF-1/CXCL12 in samples. No significant cross-reactivity or interference between Mouse SDF-1/CXCL12 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Highest expression levels detected in kidney, liver, spleen and muscle. Isoform Alpha is expressed ubiquitously but at varying levels, while isoform Beta displays tissue-specific expression, with expression detected in kidney, liver, heart, spleen and muscle but not in lung, colon, brain, skin and stomach. |
| Function | Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development.required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells. |
| Protein Name | Stromal Cell-Derived Factor 1Sdf-112-O-Tetradecanoylphorbol 13-Acetate Repressed Protein 1Tpar1C-X-C Motif Chemokine 12Pre-B Cell Growth-Stimulating FactorPbsfThymic Lymphoma Cell-Stimulating FactorTlsf |
| Database Links | Reactome: R-MMU-376176Reactome: -MMU-380108Reactome: -MMU-418594 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Stromal Cell-Derived Factor 1 ELISA kitSdf-1 ELISA kit12-O-Tetradecanoylphorbol 13-Acetate Repressed Protein 1 ELISA kitTpar1 ELISA kitC-X-C Motif Chemokine 12 ELISA kitPre-B Cell Growth-Stimulating Factor ELISA kitPbsf ELISA kitThymic Lymphoma Cell-Stimulating Factor ELISA kitTlsf ELISA kitCxcl12 ELISA kitSdf1 ELISA kit |
| Specificity | This kit recognizes Mouse SDF-1/CXCL12 in samples. No significant cross-reactivity or interference between Mouse SDF-1/CXCL12 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-101 | 95 |
| EDTA plasma (n=8) | 94-107 | 101 |
| Cell culture media (n=8) | 90-108 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 233.37 | 801.03 | 2381.44 | 234.06 | 864.84 | 2213.04 |
| Standard deviation | 12.93 | 32.20 | 79.54 | 15.07 | 39.26 | 77.68 |
| CV (%) | 5.54 | 4.02 | 3.34 | 6.44 | 4.54 | 3.51 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
