• Mouse Sdc1 (Syndecan-1) Sandwich ELISA (STJE0005223)

Mouse Sdc1 (Syndecan-1) Sandwich ELISA (STJE0005223)

SKU:
STJE0005223

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This Sdc1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Sdc1
Gene ID: 20969
Uniprot ID: SDC1_MOUSE
Specificity: This kit recognizes Mouse SDC1 in samples. No significant cross-reactivity or interference between Mouse SDC1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP. Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway.
Protein Name Syndecan-1
Synd1
Cd Antigen Cd138
Database Links Reactome: R-MMU-1971475
Reactome: -MMU-2022928
Reactome: -MMU-2024096
Reactome: -MMU-202733
Reactome: -MMU-3000170
Reactome: -MMU-449836
Reactome: -MMU-975634
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Secreted
Extracellular Exosome
Shedding Of The Ectodomain Produces A Soluble Form
Alternative ELISA Names Syndecan-1 ELISA kit
Synd1 ELISA kit
Cd Antigen Cd138 ELISA kit
Sdc1 ELISA kit
Synd-1 ELISA kit
Synd1 ELISA kit
Specificity This kit recognizes Mouse SDC1 in samples. No significant cross-reactivity or interference between Mouse SDC1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-100 94
EDTA plasma (n=8) 92-106 100
Cell culture media (n=8) 90-106 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.46 1.00 4.46 0.48 1.10 4.68
Standard deviation 0.03 0.04 0.20 0.03 0.06 0.16
CV (%) 6.52 4.00 4.48 6.25 5.45 3.42
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance