| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This S100a8 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
S100a8 |
| Gene ID: |
20201 |
| Uniprot ID: |
S10A8_MOUSE |
| Specificity: |
This kit recognizes Mouse S100A8 in samples. No significant cross-reactivity or interference between Mouse S100A8 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Protein Name | Protein S100-A8Calgranulin-AChemotactic Cytokine Cp-10Leukocyte L1 Complex Light ChainMigration Inhibitory Factor-Related Protein 8Mrp-8P8Pro-Inflammatory S100 CytokineS100 Calcium-Binding Protein A8 |
| Database Links | Reactome: R-MMU-5668599Reactome: -MMU-5686938Reactome: -MMU-6798695Reactome: -MMU-6799990 |
| Cellular Localisation | SecretedCytoplasmCytoskeletonCell MembranePeripheral Membrane ProteinPredominantly Localized In The CytoplasmUpon Elevation Of The Intracellular Calcium LevelTranslocated From The Cytoplasm To The Cytoskeleton And The Cell MembraneUpon Neutrophil Activation Or Endothelial Adhesion Of MonocytesIs Secreted Via A Microtubule-MediatedAlternative Pathway |
| Alternative ELISA Names | Protein S100-A8 ELISA kitCalgranulin-A ELISA kitChemotactic Cytokine Cp-10 ELISA kitLeukocyte L1 Complex Light Chain ELISA kitMigration Inhibitory Factor-Related Protein 8 ELISA kitMrp-8 ELISA kitP8 ELISA kitPro-Inflammatory S100 Cytokine ELISA kitS100 Calcium-Binding Protein A8 ELISA kitS100a8 ELISA kitCaga ELISA kitMrp8 ELISA kit |
| Specificity | This kit recognizes Mouse S100A8 in samples. No significant cross-reactivity or interference between Mouse S100A8 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-97 | 93 |
| EDTA plasma (n=8) | 83-95 | 90 |
| Cell culture media (n=8) | 90-103 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 188.64 | 414.53 | 1934.43 | 195.77 | 446.65 | 1946.95 |
| Standard deviation | 11.11 | 20.60 | 92.08 | 11.39 | 20.64 | 78.07 |
| CV (%) | 5.89 | 4.97 | 4.76 | 5.82 | 4.62 | 4.01 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
