| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25-2000pg/mL |
| Short Description: |
This RANTES Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Ccl5 |
| Gene ID: |
20304 |
| Uniprot ID: |
CCL5_MOUSE |
| Specificity: |
This kit recognizes Mouse RANTES in samples. No significant cross-reactivity or interference between Mouse RANTES and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells. |
| Protein Name | C-C Motif Chemokine 5MurantesSis-DeltaSmall-Inducible Cytokine A5T-Cell-Specific Protein Rantes |
| Database Links | Reactome: R-MMU-380108Reactome: -MMU-418594 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | C-C Motif Chemokine 5 ELISA kitMurantes ELISA kitSis-Delta ELISA kitSmall-Inducible Cytokine A5 ELISA kitT-Cell-Specific Protein Rantes ELISA kitCcl5 ELISA kitScya5 ELISA kit |
| Specificity | This kit recognizes Mouse RANTES in samples. No significant cross-reactivity or interference between Mouse RANTES and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-109 | 101 |
| EDTA plasma (n=8) | 95-109 | 101 |
| Cell culture media (n=8) | 90-103 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 108.68 | 254.58 | 815.44 | 99.16 | 269.40 | 806.85 |
| Standard deviation | 7.48 | 10.79 | 39.63 | 6.60 | 10.99 | 41.47 |
| CV (%) | 6.88 | 4.24 | 4.86 | 6.66 | 4.08 | 5.14 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
