| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.061ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This PLN Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Pln |
| Gene ID: |
18821 |
| Uniprot ID: |
PPLA_MOUSE |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in testis (at protein level). |
| Post Translational Modifications | Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Palmitoylated by ZDHHC16, promoting formation of the homopentamer. In elongated spermatids, proteolytically cleaved by SPPL2C which modulates intracellular Ca(2+) homeostasis. |
| Function | Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. Controls intracellular Ca(2+) levels in elongated spermatids. May play a role in germ cell differentiation. |
| Protein Name | Cardiac PhospholambanPlb |
| Database Links | Reactome: R-MMU-5578775Reactome: -MMU-936837 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinSarcoplasmic Reticulum MembraneMitochondrion MembraneMembraneColocalizes With Hax1 At The Endoplasmic ReticulumColocalizes With Dmpk A The Sarcoplasmic Reticulum |
| Alternative ELISA Names | Cardiac Phospholamban ELISA kitPlb ELISA kitPln ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
