Mouse PF4 (Platelet Factor 4) Sandwich ELISA (STJE0019780)

SKU:
STJE0019780

Shipping:
Free Shipping
Current Stock:
Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.38ng/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This PF4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Pf4
Gene ID: 56744
Uniprot ID: PLF4_MOUSE
Specificity: This kit recognizes Mouse PF4 in samples. No significant cross-reactivity or interference between Mouse PF4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.
Protein Name Platelet Factor 4
Pf-4
C-X-C Motif Chemokine 4
Database Links Reactome: R-MMU-114608
Reactome: -MMU-140875
Reactome: -MMU-202733
Reactome: -MMU-380108
Reactome: -MMU-418594
Cellular Localisation Secreted
Alternative ELISA Names Platelet Factor 4 ELISA kit
Pf-4 ELISA kit
C-X-C Motif Chemokine 4 ELISA kit
Pf4 ELISA kit
Cxcl4 ELISA kit
Scyb4 ELISA kit
Specificity This kit recognizes Mouse PF4 in samples. No significant cross-reactivity or interference between Mouse PF4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-101 95
EDTA plasma (n=8) 84-99 91
Cell culture media (n=8) 84-98 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 103.27 312.54 739.29 111.53 312.50 747.32
Standard deviation 7.20 13.69 32.23 5.75 16.09 36.54
CV (%) 6.97 4.38 4.36 5.16 5.15 4.89
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance