| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
21.3pg/mL |
| Detection Limit: |
78.1-5000pg/mL |
| Short Description: |
This PENK Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
Penk |
| Gene ID: |
18619 |
| Uniprot ID: |
PENK_MOUSE |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Spermatogenic and somatic cells. |
| Post Translational Modifications | Proenkephalin-A is cleaved by CTSL to generate Met-enkephalin. Met-enkephalin: Processed and degraded by ACE. Leu-enkephalin: Processed and degraded by ACE. Met-enkephalin-Arg-Ser-Leu: Probably cleaved by ACE. Met-enkephalin-Arg-Phe: Processed by ACE to generate Met-enkephalin in the nucleus accumbens of the brain. The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. |
| Function | Met-enkephalin: Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Leu-enkephalin: Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Met-enkephalin-Arg-Phe: Met-enkephalin-Arg-Phe neuropeptide acts as a strong ligand of Mu-type opioid receptor OPRM1. Met-enkephalin-Arg-Phe-binding to OPRM1 in the nucleus accumbens of the brain increases activation of OPRM1, leading to long-term synaptic depression of glutamate release. PENK(114-133): Increases glutamate release in the striatum and decreases GABA concentration in the striatum. PENK(238-259): Increases glutamate release in the striatum. |
| Protein Name | Proenkephalin-A Cleaved Into - Synenkephalin - Met-EnkephalinOpioid Growth FactorOgf - Penk(114-133 - Penk(143-184 - Met-Enkephalin-Arg-Ser-Leu - Leu-Enkephalin - Penk(238-259 - Met-Enkephalin-Arg-Phe |
| Database Links | Reactome: R-MMU-375276Reactome: -MMU-381426Reactome: -MMU-418594Reactome: -MMU-8957275 |
| Cellular Localisation | Cytoplasmic VesicleSecretory VesicleChromaffin Granule LumenSecreted |
| Alternative ELISA Names | Proenkephalin-A Cleaved Into - Synenkephalin - Met-Enkephalin ELISA kitOpioid Growth Factor ELISA kitOgf - Penk(114-133 - Penk(143-184 - Met-Enkephalin-Arg-Ser-Leu - Leu-Enkephalin - Penk(238-259 - Met-Enkephalin-Arg-Phe ELISA kitPenk ELISA kitPenk1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
