• Mouse PAR2 (Protease Activated Receptor 2) Sandwich ELISA Kit Ready-To-Use (STJE0017664)

Mouse PAR2 (Protease Activated Receptor 2) Sandwich ELISA Kit Ready-To-Use (STJE0017664)

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STJE0017664

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Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.053ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This PAR2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: F2rl1
Gene ID: 14063
Uniprot ID: PAR2_MOUSE
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity
Post Translational Modifications A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand. Activating serine proteases include trypsin, mast cell tryptase, coagulation factors VII and Xa, myeloblastin/PRTN3 and membrane-type serine protease 1/ST14. Proposed subsequent cleavage by serine proteases is leading to receptor deactivation and include neutrophil elastase and cathepsin G. At least in part, implicated proteases are also shown to activate the receptor.the glycosylation status of the receptor is thought to contribute to the difference. N-glycosylated and sialylated. Multiple phosphorylated on serine and threonine residues in the cytoplasmic region upon receptor activation.required for receptor desensitization and recruitment of beta-arrestin. Monoubiquitinated by Cbl at the plasma membrane and in early endosomes.not required for receptor endocytosis but for translocation to late endosomes or lysosomes. Deubiquitination involves Stambp and Usp8.required for lysosomal trafficking and receptor degradation.
Function Receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Can also be transactivated by cleaved F2r/Par1. Involved in modulation of inflammatory responses and regulation of innate and adaptive immunity, and acts as a sensor for proteolytic enzymes generated during infection. Generally is promoting inflammation. Can signal synergistically with Tlr4 and probably Tlr2 in inflammatory responses and modulates Tlr3 signaling. Has a protective role in establishing the endothelial barrier.the activity involves coagulation factor X. Regulates endothelial cell barrier integrity during neutrophil extravasation, probably following proteolytic cleavage by PRTN3. Proposed to have a bronchoprotective role in airway epithelium, but also shown to compromise the airway epithelial barrier by interrupting E-cadherin adhesion. Involved in the regulation of vascular tone.activation results in hypotension presumably mediated by vasodilation. Associates with a subset of G proteins alpha subunits such as GNAQ, GNA11, GNA14, GNA12 and GNA13, but probably not with G(o)-alpha, G(i) subunit alpha-1 and G(i) subunit alpha-2. Believed to be a class B receptor which internalizes as a complex with arrestin and traffic with it to endosomal vesicles, presumably as desensitized receptor, for extended periods of time. Mediates inhibition of TNF-alpha stimulated JNK phosphorylation via coupling to GNAQ and GNA11.the function involves dissociation of Ripk1 and Tradd from Tnfr1. Mediates phosphorylation of nuclear factor NF-kappa-B RELA subunit at 'Ser-536'.the function involves Ikbkb and is predominantly independent of G proteins. Involved in cellular migration. Involved in cytoskeletal rearrangement and chemotaxis through beta-arrestin-promoted scaffolds.the function is independent of GNAQ and GNA11 and involves promotion of cofilin dephosphorylation and actin filament severing. Induces redistribution of Cops5 from the plasma membrane to the cytosol and activation of the JNK cascade is mediated by Cops5. Involved in the recruitment of leukocytes to the sites of inflammation and is the major PAR receptor capable of modulating eosinophil function such as pro-inflammatory cytokine secretion, superoxide production and degranulation. During inflammation promotes dendritic cell maturation, trafficking to the lymph nodes and subsequent T-cell activation. Involved in antimicrobial response of innate immune cells.activation enhances phagocytosis of Gram-positive and killing of Gram-negative bacteria. Acts synergistically with interferon-gamma in enhancing antiviral responses.
Protein Name Proteinase-Activated Receptor 2
Par-2
Coagulation Factor Ii Receptor-Like 1
G-Protein Coupled Receptor 11
Thrombin Receptor-Like 1
Database Links Reactome: R-MMU-375276
Reactome: -MMU-416476
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative ELISA Names Proteinase-Activated Receptor 2 ELISA kit
Par-2 ELISA kit
Coagulation Factor Ii Receptor-Like 1 ELISA kit
G-Protein Coupled Receptor 11 ELISA kit
Thrombin Receptor-Like 1 ELISA kit
F2rl1 ELISA kit
Gpcr11 ELISA kit
Gpr11 ELISA kit
Par2 ELISA kit
output

Information sourced from Uniprot.org

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