Mouse OPG (Osteoprotegerin) Sandwich ELISA (STJE0019752)

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STJE0019752

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 62.87pg/mL
Detection Limit: 125-8000pg/mL
Short Description: This OPG Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Tnfrsf11b
Gene ID: 18383
Uniprot ID: TR11B_MOUSE
Specificity: This kit recognizes Mouse OPG in samples. No significant cross-reactivity or interference between Mouse OPG and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
Protein Name Tumor Necrosis Factor Receptor Superfamily Member 11b
Osteoclastogenesis Inhibitory Factor
Osteoprotegerin
Database Links Reactome: R-MMU-5669034
Cellular Localisation Secreted
Alternative ELISA Names Tumor Necrosis Factor Receptor Superfamily Member 11b ELISA kit
Osteoclastogenesis Inhibitory Factor ELISA kit
Osteoprotegerin ELISA kit
Tnfrsf11b ELISA kit
Ocif ELISA kit
Opg ELISA kit
Specificity This kit recognizes Mouse OPG in samples. No significant cross-reactivity or interference between Mouse OPG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-102 96
EDTA plasma (n=8) 88-100 94
Cell culture media (n=8) 87-102 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 437.57 693.40 3412.09 452.19 753.49 3342.64
Standard deviation 29.80 37.10 172.31 29.48 33.30 105.29
CV (%) 6.81 5.35 5.05 6.52 4.42 3.15
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance