• Mouse Nphs1 (Nephrin) Sandwich ELISA (STJE0005178)

Mouse Nphs1 (Nephrin) Sandwich ELISA (STJE0005178)

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STJE0005178

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This Nphs1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Nphs1
Gene ID: 54631
Uniprot ID: NPHN_MOUSE
Specificity: This kit recognizes Mouse NPHN in samples. No significant cross-reactivity or interference between Mouse NPHN and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylated at Tyr-1208 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. Tyrosine phosphorylation is reduced by high glucose levels. Dephosphorylated by tensin TNS2 which leads to reduced binding of NPHN1 to PIK3R1.
Function Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
Protein Name Nephrin
Renal Glomerulus-Specific Cell Adhesion Receptor
Database Links Reactome: R-MMU-373753
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Located At Podocyte Slit Diaphragm Between Podocyte Foot Processes
Alternative ELISA Names Nephrin ELISA kit
Renal Glomerulus-Specific Cell Adhesion Receptor ELISA kit
Nphs1 ELISA kit
Nphn ELISA kit
Specificity This kit recognizes Mouse NPHN in samples. No significant cross-reactivity or interference between Mouse NPHN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 95
EDTA plasma (n=8) 92-104 99
Cell culture media (n=8) 90-105 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.52 1.00 4.89 0.49 0.97 4.86
Standard deviation 0.03 0.04 0.15 0.03 0.06 0.22
CV (%) 5.77 4.00 3.07 6.12 6.19 4.53
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance