| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This Nphs1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Nphs1 |
| Gene ID: |
54631 |
| Uniprot ID: |
NPHN_MOUSE |
| Specificity: |
This kit recognizes Mouse NPHN in samples. No significant cross-reactivity or interference between Mouse NPHN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated at Tyr-1208 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. Tyrosine phosphorylation is reduced by high glucose levels. Dephosphorylated by tensin TNS2 which leads to reduced binding of NPHN1 to PIK3R1. |
| Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. |
| Protein Name | NephrinRenal Glomerulus-Specific Cell Adhesion Receptor |
| Database Links | Reactome: R-MMU-373753 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinLocated At Podocyte Slit Diaphragm Between Podocyte Foot Processes |
| Alternative ELISA Names | Nephrin ELISA kitRenal Glomerulus-Specific Cell Adhesion Receptor ELISA kitNphs1 ELISA kitNphn ELISA kit |
| Specificity | This kit recognizes Mouse NPHN in samples. No significant cross-reactivity or interference between Mouse NPHN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-100 | 95 |
| EDTA plasma (n=8) | 92-104 | 99 |
| Cell culture media (n=8) | 90-105 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.52 | 1.00 | 4.89 | 0.49 | 0.97 | 4.86 |
| Standard deviation | 0.03 | 0.04 | 0.15 | 0.03 | 0.06 | 0.22 |
| CV (%) | 5.77 | 4.00 | 3.07 | 6.12 | 6.19 | 4.53 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
