• Mouse Nfe2l2 (Nuclear factor erythroid 2-related factor 2) Sandwich ELISA (STJE0005231)

Mouse Nfe2l2 (Nuclear factor erythroid 2-related factor 2) Sandwich ELISA (STJE0005231)

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STJE0005231

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This Nfe2l2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Nfe2l2
Gene ID: 18024
Uniprot ID: NF2L2_MOUSE
Specificity: This kit recognizes Mouse NFE2L2 in samples. No significant cross-reactivity or interference between Mouse NFE2L2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Ubiquitinated in the cytoplasm by the BCR(KEAP1) E3 ubiquitin ligase complex leading to its degradation. In response to oxidative stress, electrophile metabolites, such as sulforaphane, modify KEAP1, leading to inhibit activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and activity. In response to autophagy, the BCR(KEAP1) complex is inactivated. Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. Acetylation at Lys-588 and Lys-591 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence. Glycation impairs transcription factor activity by preventing heterodimerization with small Maf proteins. Deglycation by FN3K restores activity.
Function Transcription factor that plays a key role in the response to oxidative stress: binds to antioxidant response (ARE) elements present in the promoter region of many cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression, thereby neutralizing reactive electrophiles. In normal conditions, ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex. In response to oxidative stress, electrophile metabolites inhibit activity of the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2, heterodimerization with one of the small Maf proteins and binding to ARE elements of cytoprotective target genes. The NFE2L2/NRF2 pathway is also activated in response to selective autophagy: autophagy promotes interaction between KEAP1 and SQSTM1/p62 and subsequent inactivation of the BCR(KEAP1) complex, leading to NFE2L2/NRF2 nuclear accumulation and expression of cytoprotective genes. May also be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Also plays an important role in the regulation of the innate immune response. It is a critical regulator of the innate immune response and survival during sepsis by maintaining redox homeostasis and restraint of the dysregulation of pro-inflammatory signaling pathways like MyD88-dependent and -independent and TNF-alpha signaling. Suppresses macrophage inflammatory response by blocking pro-inflammatory cytokine transcription and the induction of IL6. Binds to the proximity of pro-inflammatory genes in macrophages and inhibits RNA Pol II recruitment. The inhibition is independent of the Nrf2-binding motif and reactive oxygen species level. Represses antiviral cytosolic DNA sensing by suppressing the expression of the adapter protein STING1 and decreasing responsiveness to STING1 agonists while increasing susceptibility to infection with DNA viruses.
Protein Name Nuclear Factor Erythroid 2-Related Factor 2
Nf-E2-Related Factor 2
Nfe2-Related Factor 2
Nuclear Factor - Erythroid Derived 2 - Like 2
Database Links Reactome: R-MMU-8951664
Reactome: -MMU-9755511
Reactome: -MMU-9759194
Reactome: -MMU-9762114
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Cytosolic Under Unstressed Conditions: Ubiquitinated And Degraded By The Bcr(Keap1) E3 Ubiquitin Ligase Complex
Translocates Into The Nucleus Upon Induction By Electrophilic Agents That Inactivate The Bcr(Keap1) E3 Ubiquitin Ligase Complex
Alternative ELISA Names Nuclear Factor Erythroid 2-Related Factor 2 ELISA kit
Nf-E2-Related Factor 2 ELISA kit
Nfe2-Related Factor 2 ELISA kit
Nuclear Factor - Erythroid Derived 2 - Like 2 ELISA kit
Nfe2l2 ELISA kit
Nrf2 ELISA kit
Specificity This kit recognizes Mouse NFE2L2 in samples. No significant cross-reactivity or interference between Mouse NFE2L2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-101 93
EDTA plasma (n=8) 91-102 96
Cell culture media (n=8) 93-107 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.09 2.00 7.44 1.20 2.05 6.84
Standard deviation 0.06 0.09 0.40 0.07 0.11 0.34
CV (%) 5.50 4.50 5.38 5.83 5.37 4.97
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance