| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.055ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This NFATC1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Nfatc1Uniprot ID=NFAC1_MOUSE" |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in the submandibular gland (at protein level). Expressed in basal epidermal cells (at protein level). Expressed in spleen, lung, skeletal muscle, thymus and skin. Weakly expressed in heart, brain, liver and kidney. Not expressed in testis. Expressed in osteoclasts. Also expressed during TNFSF11/RANKL-induced differentiation of bone marrow-derived macrophages to osteoclasts. |
| Post Translational Modifications | Phosphorylated by NFATC-kinase and GSK3B.phosphorylation induces NFATC1 nuclear exit and dephosphorylation by calcineurin promotes nuclear import. Phosphorylation by PKA and DYRK2 negatively modulates nuclear accumulation, and promotes subsequent phosphorylation by GSK3B or casein kinase 1. |
| Function | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function. |
| Protein Name | Nuclear Factor Of Activated T-Cells - Cytoplasmic 1Nf-Atc1Nfatc1Nfat Transcription Complex Cytosolic ComponentNf-AtcNfatc |
| Database Links | |
| Cellular Localisation | CytoplasmNucleusCytoplasmic For The Phosphorylated Form And Nuclear After Activation That Is Controlled By Calcineurin-Mediated DephosphorylationRapid Nuclear Exit Of Nfatc Is Thought To Be One Mechanism By Which Cells Distinguish Between Sustained And Transient Calcium SignalsThe Subcellular Localization Of Nfatc Plays A Key Role In The Regulation Of Gene TranscriptionNuclear Translocation Of Nfatc1 Is Enhanced In The Presence Of Tnfsf11Nuclear Translocation Is Decreased In The Presence Of Fbn1 Which Can Bind And Sequester Tnfsf11 |
| Alternative ELISA Names | Nuclear Factor Of Activated T-Cells - Cytoplasmic 1 ELISA kitNf-Atc1 ELISA kitNfatc1 ELISA kitNfat Transcription Complex Cytosolic Component ELISA kitNf-Atc ELISA kitNfatc ELISA kitNfatc1 ELISA kitNfat2 ELISA kitNfatc ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
