| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This Mydgf Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Mydgf |
| Gene ID: |
28106 |
| Uniprot ID: |
MYDGF_MOUSE |
| Specificity: |
This kit recognizes Mouse IL-25 in samples. No significant cross-reactivity or interference between Mouse IL-25 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway. |
| Protein Name | Myeloid-Derived Growth FactorMydgf |
| Cellular Localisation | SecretedEndoplasmic Reticulum-Golgi Intermediate CompartmentEndoplasmic ReticulumGolgi ApparatusThe C-Terminal Rtel Motif May Provide Retention In The Endoplasmic Reticulum |
| Alternative ELISA Names | Myeloid-Derived Growth Factor ELISA kitMydgf ELISA kitMydgf ELISA kitD17Wsu104e ELISA kit |
| Specificity | This kit recognizes Mouse IL-25 in samples. No significant cross-reactivity or interference between Mouse IL-25 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-99 | 91 |
| EDTA plasma (n=8) | 89-103 | 96 |
| Cell culture media (n=8) | 90-105 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 50.94 | 140.25 | 422.86 | 55.24 | 134.27 | 436.28 |
| Standard deviation | 3.49 | 6.68 | 22.79 | 3.65 | 5.63 | 21.16 |
| CV (%) | 6.85 | 4.76 | 5.39 | 6.61 | 4.19 | 4.85 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
