Mouse MCP-2 (Monocyte Chemotactic Protein 2) Sandwich ELISA (STJE0019983)

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STJE0019983

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 10.45 pg/mL
Detection Limit: 31.25-2000 pg/mL
Short Description: This MCP-2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Ccl8
Gene ID: 20307
Uniprot ID: CCL8_MOUSE
Specificity: This kit recognizes Mouse MCP-2 in samples. No significant cross-reactivity or interference between Mouse MCP-2 and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Function Chemotactic factor that attracts monocytes. This protein can bind heparin.
Protein Name C-C Motif Chemokine 8
Monocyte Chemoattractant Protein 2
Monocyte Chemotactic Protein 2
Mcp-2
Small-Inducible Cytokine A8
Cellular Localisation Secreted
Alternative ELISA Names C-C Motif Chemokine 8 ELISA kit
Monocyte Chemoattractant Protein 2 ELISA kit
Monocyte Chemotactic Protein 2 ELISA kit
Mcp-2 ELISA kit
Small-Inducible Cytokine A8 ELISA kit
Ccl8 ELISA kit
Mcp2 ELISA kit
Scya8 ELISA kit
Specificity This kit recognizes Mouse MCP-2/CCL8 in samples. No significant cross-reactivity or interference between Mouse MCP-2/CCL8 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-102 97
EDTA plasma (n=8) 92-104 97
Cell culture media (n=8) 95-106 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 95.86 289.60 760.10 89.64 293.67 766.29
Standard deviation 5.25 12.19 36.71 6.01 15.33 38.31
CV (%) 5.48 4.21 4.83 6.71 5.22 5.00
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance