| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This Mbp Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Mbp |
| Gene ID: |
17196 |
| Uniprot ID: |
MBP_MOUSE |
| Specificity: |
This kit recognizes Mouse MBP in samples. No significant cross-reactivity or interference between Mouse MBP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | As in other animals, several charge isomers may be produced as a result of optional post-translational modifications, such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues. Methylated on arginine residues.decreases with the age of the animal, making MBP more cationic. Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1. Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells. |
| Function | The classic group of MBP isoforms (isoform 4-isoform 13) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. |
| Protein Name | Myelin Basic ProteinMbpMyelin A1 Protein |
| Cellular Localisation | Isoform 13: Myelin MembranePeripheral Membrane ProteinCytoplasmic SideIsoform 12: Myelin MembraneIsoform 11: Myelin MembraneIsoform 10: Myelin MembraneIsoform 9: Myelin MembraneIsoform 8: Myelin MembraneIsoform 7: Myelin MembraneIsoform 6: Myelin MembraneIsoform 5: Myelin MembraneIsoform 4: Myelin MembraneIsoform 3: CytoplasmNucleusIsoform 2: CytoplasmIsoform 1: Cytoplasm |
| Alternative ELISA Names | Myelin Basic Protein ELISA kitMbp ELISA kitMyelin A1 Protein ELISA kitMbp ELISA kitShi ELISA kit |
| Specificity | This kit recognizes Mouse MBP in samples. No significant cross-reactivity or interference between Mouse MBP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-104 | 96 |
| EDTA plasma (n=8) | 95-106 | 100 |
| Cell culture media (n=8) | 87-97 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.54 | 0.92 | 3.70 | 0.54 | 0.95 | 4.02 |
| Standard deviation | 0.03 | 0.05 | 0.18 | 0.03 | 0.04 | 0.13 |
| CV (%) | 5.56 | 5.43 | 4.86 | 5.56 | 4.21 | 3.23 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
