Mouse LEP (Leptin) Sandwich ELISA (STJE0019747)

SKU:
STJE0019747

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.38ng/mL
Detection Limit: 0.63-40pg/mL
Short Description: This LEP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Lep
Gene ID: 16846
Uniprot ID: LEP_MOUSE
Specificity: This kit recognizes Mouse LEP in samples. No significant crossreactivity or interference between Mouse LEP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Leptin
Obesity Factor
Database Links Reactome: R-MMU-381771
Reactome: -MMU-422085
Cellular Localisation Secreted
Alternative ELISA Names Leptin ELISA kit
Obesity Factor ELISA kit
Lep ELISA kit
Ob ELISA kit
Specificity This kit recognizes Mouse LEP in samples. No significant cross-reactivity or interference between Mouse LEP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-108 100
EDTA plasma (n=8) 93-108 101
Cell culture media (n=8) 90-105 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.13 5.51 15.51 2.27 5.09 16.44
Standard deviation 0.11 0.24 0.79 0.13 0.28 0.61
CV (%) 5.15 4.38 5.12 5.93 5.59 3.73
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance