| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
6.9pg/mL |
| Detection Limit: |
15.6-1000pg/mL |
| Short Description: |
This LCAT Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Lcat |
| Gene ID: |
16816 |
| Uniprot ID: |
LCAT_MOUSE |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Detected in blood plasma. Produced and secreted by astrocytes (at protein level). Abundantly expressed in liver, brain and testis with highest levels in liver. In the brain, found in cerebellum, cerebral cortex, hippocampus and brain stem. Located to neurons and neuroglia. |
| Post Translational Modifications | |
| Function | Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF. Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters. |
| Protein Name | Phosphatidylcholine-Sterol Acyltransferase1-Alkyl-2-Acetylglycerophosphocholine EsteraseLecithin-Cholesterol AcyltransferasePhospholipid-Cholesterol AcyltransferasePlatelet-Activating Factor AcetylhydrolasePaf Acetylhydrolase |
| Database Links | Reactome: R-MMU-8964058 |
| Cellular Localisation | SecretedSecreted Into Blood PlasmaProduced In Astrocytes And Secreted Into Cerebral Spinal Fluid (Csf) |
| Alternative ELISA Names | Phosphatidylcholine-Sterol Acyltransferase ELISA kit1-Alkyl-2-Acetylglycerophosphocholine Esterase ELISA kitLecithin-Cholesterol Acyltransferase ELISA kitPhospholipid-Cholesterol Acyltransferase ELISA kitPlatelet-Activating Factor Acetylhydrolase ELISA kitPaf Acetylhydrolase ELISA kitLcat ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
