Applications: |
ELISA |
Reactivity: |
Mouse |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
0.94ng/mL |
Detection Limit: |
1.56~100ng/mL |
Short Description: |
This Kng1 Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
2.5h |
Detection: |
Colormetric |
Gene Symbol: |
Kng1 |
Gene ID: |
16644 |
Uniprot ID: |
KNG1_MOUSE |
Specificity: |
This kit recognizes Mouse BK in samples. No significant cross-reactivity or interference between Mouse BK and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Post Translational Modifications | Bradykinin is released from kininogen by plasma kallikrein. Phosphorylated by FAM20C in the extracellular medium. Bradykinin: Bradykinin is inactivated by ACE, which removes the dipeptide Arg-Phe from its C-terminus. |
Function | Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII.HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting. Bradykinin: The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects: (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action). |
Protein Name | Kininogen-1 Cleaved Into - Kininogen-1 Heavy Chain - Bradykinin - Kininogen-1 Light Chain |
Cellular Localisation | SecretedExtracellular Space |
Alternative ELISA Names | Kininogen-1 Cleaved Into - Kininogen-1 Heavy Chain - Bradykinin - Kininogen-1 Light Chain ELISA kitKng1 ELISA kitKng ELISA kit |
Specificity | This kit recognizes BK in samples. No significant cross-reactivity or interference between BK and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 92-107 | 99 |
EDTA plasma(n=8) | 89-100 | 94 |
Cell culture media(n=8) | 92-103 | 97 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 5.00 | 15.50 | 44.10 | 4.60 | 15.20 | 45.50 |
Standard deviation | 0.30 | 0.70 | 2.10 | 0.20 | 0.80 | 1.60 |
CV (%) | 6.00 | 4.52 | 4.76 | 4.35 | 5.26 | 3.52 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance