| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
5.8pg/mL |
| Detection Limit: |
15.6-1000pg/mL |
| Short Description: |
This KLF1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Klf1Uniprot ID=KLF1_MOUSE" |
| Sample Type: |
serum, plasma or other biological fluids. |
| Tissue Specificity | Erythroid-specific. Only expressed in bone marrow and spleen. |
| Post Translational Modifications | Acetylated.can be acetylated on both Lys-270 and Lys-288. Acetylation on Lys-270 (by CBP) appears to be the major site affecting EKLF transactivation activity. Sumoylated.sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex. Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity. |
| Function | Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. Probably serves as a general switch factor for erythroid development. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation. |
| Protein Name | Krueppel-Like Factor 1Erythroid Krueppel-Like Transcription FactorEklf |
| Database Links | |
| Cellular Localisation | NucleusColocalizes With Sumo1 In Nuclear Speckles |
| Alternative ELISA Names | Krueppel-Like Factor 1 ELISA kitErythroid Krueppel-Like Transcription Factor ELISA kitEklf ELISA kitKlf1 ELISA kitElkf ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
