Mouse Kl (Klotho) Sandwich ELISA (STJE0005367)

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STJE0005367

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This Kl Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Kl
Gene ID: 16591
Uniprot ID: KLOT_MOUSE
Specificity: This kit recognizes Mouse Klotho in samples. No significant cross-reactivity or interference between Mouse Klotho and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N-glycosylated.
Function May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
Protein Name Klotho Cleaved Into - Klotho Peptide
Database Links Reactome: R-MMU-109704
Reactome: -MMU-1257604
Reactome: -MMU-190374
Reactome: -MMU-5654219
Reactome: -MMU-5654687
Reactome: -MMU-5654688
Reactome: -MMU-5654689
Reactome: -MMU-5654693
Reactome: -MMU-5654726
Reactome: -MMU-5673001
Reactome: -MMU-6811558
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Apical Cell Membrane
Isoform 1 Shedding Leads To A Soluble Peptide
Isoform 2: Secreted
Klotho Peptide: Secreted
Alternative ELISA Names Klotho Cleaved Into - Klotho Peptide ELISA kit
Kl ELISA kit
Specificity This kit recognizes Mouse Klotho in samples. No significant cross-reactivity or interference between Mouse Klotho and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-100 97
EDTA plasma (n=8) 87-98 94
Cell culture media (n=8) 89-99 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 42.38 103.30 441.20 44.75 107.45 409.89
Standard deviation 2.20 4.75 20.65 2.51 4.91 22.38
CV (%) 5.19 4.60 4.68 5.61 4.57 5.46
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance