| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This Il24 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Il24 |
| Gene ID: |
93672 |
| Uniprot ID: |
IL24_MOUSE |
| Specificity: |
This kit recognizes Mouse IL-24 in samples. No significant cross-reactivity or interference between Mouse IL-24 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Glycosylated. Ubiquitination at Lys-97 promotes proteasomal degradation. |
| Function | Multifunctional cytokine mainly produced by T-cells that plays a regulatory role in immune response, tissue homeostasis, host defense, and oncogenesis. Possesses antiviral functions and induces the type I intereferon response during influenza infection. Signals through two receptor complexes IL20RA/IL20RB or IL20RB/IL22RA1. In turn, stimulates the JAK1-STAT3 and MAPK pathways and promotes the secretion of pro-inflammatory mediators including IL8 and MMP1. Intracellularly, maintains endoplasmic reticulum homeostasis by restricting the eIF2alpha-CHOP pathway-mediated stress signal. In addition, acts as a quality control mechanism for the ubiquitin proteasome system by alerting the cell to proteasome dysfunction through activation of PKR/EIF2AK2. |
| Protein Name | Interleukin-24Il-24Il-4-Induced Secreted ProteinMelanoma Differentiation-Associated Gene 7 ProteinMda-7Th2-Specific Cytokine Fisp |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Interleukin-24 ELISA kitIl-24 ELISA kitIl-4-Induced Secreted Protein ELISA kitMelanoma Differentiation-Associated Gene 7 Protein ELISA kitMda-7 ELISA kitTh2-Specific Cytokine Fisp ELISA kitIl24 ELISA kitMda7 ELISA kit |
| Specificity | This kit recognizes Mouse IL-24 in samples. No significant cross-reactivity or interference between Mouse IL-24 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-105 | 98 |
| EDTA plasma (n=8) | 93-105 | 98 |
| Cell culture media (n=8) | 88-102 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 97.60 | 182.50 | 915.30 | 106.40 | 176.00 | 837.70 |
| Standard deviation | 6.70 | 10.00 | 29.30 | 6.00 | 8.60 | 37.70 |
| CV (%) | 6.86 | 5.48 | 3.20 | 5.64 | 4.89 | 4.50 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
