| Host: |
HEK293 cells |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse IL-18-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 8.0. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: |
Measured by its ability to induce IFN-gamma secretion by KG1 human acute myelogenous leukemia cells in the presence of TNF-alpha (Catalog: RP00001). The ED50 for this effect is 42.93-171.72 ng/mL, corresponding to a specific activity of 0.58×104~2.3× |
| Gene Symbol: |
Il18 |
| Gene ID: |
16173 |
| Uniprot ID: |
IL18_MOUSE |
| Immunogen: |
Recombinant Mouse IL-18 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence of Mouse IL-18 fused with His tag at the C-terminus |
| Function | Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. |
| Protein Name | Interleukin-18Il-18Interferon Gamma-Inducing FactorIfn-Gamma-Inducing FactorInterleukin-1 GammaIl-1 Gamma |
| Cellular Localisation | CytoplasmSecretedThe Precursor Is CytosolicIn Response To Inflammasome-Activating SignalsCleaved And SecretedMature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) PoreIn ContrastThe Precursor Form Is Not ReleasedDue To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During MaturationThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative Protein Names | Interleukin-18 proteinIl-18 proteinInterferon Gamma-Inducing Factor proteinIfn-Gamma-Inducing Factor proteinInterleukin-1 Gamma proteinIl-1 Gamma proteinIl18 proteinIgif protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
