Mouse IL-10 (Interleukin 10) Sandwich ELISA (STJE0019745)

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STJE0019745

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.13pg/mL
Detection Limit: 15.63-1000pg/mL
Short Description: This IL-10 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Il10
Gene ID: 16153
Uniprot ID: IL10_MOUSE
Specificity: This kit recognizes Mouse IL-10 in samples. No significant cross-reactivity or interference between Mouse IL-10 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Interleukin-10
Il-10
Cytokine Synthesis Inhibitory Factor
Csif
Cellular Localisation Secreted
Alternative ELISA Names Interleukin-10 ELISA kit
Il-10 ELISA kit
Cytokine Synthesis Inhibitory Factor ELISA kit
Csif ELISA kit
Il10 ELISA kit
Il-10 ELISA kit
Specificity This kit recognizes Mouse IL-10 in samples. No significant cross-reactivity or interference between Mouse IL-10 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (50×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (50×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-107 98
EDTA plasma (n=8) 92-103 98
Cell culture media (n=8) 91-104 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 55.03 99.53 341.59 50.95 92.12 343.37
Standard deviation 3.78 4.10 10.62 3.44 4.72 16.69
CV (%) 6.86 4.12 3.11 6.75 5.12 4.86
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance