• Mouse IkBKb (Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta) Sandwich ELISA Kit Ready-To-Use (STJE0016120)

Mouse IkBKb (Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta) Sandwich ELISA Kit Ready-To-Use (STJE0016120)

SKU:
STJE0016120

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Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.054ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This IkBKb Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: Ikbkb
Gene ID: 16150
Uniprot ID: IKKB_MOUSE
Immunogen Region: Ready-To-Use
Sample Type: serum, plasma, tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity Detected in heart (at protein level). Expressed in liver, kidney and spleen.
Post Translational Modifications Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ.which enhances activity. Phosphorylated by MAP3K7/TAK1 in response to NOD1 and NOD2 signaling, promoting activation and phosphorylation of NF-kappa-B inhibitors, leading to NF-kappa-B activation. Once activated, autophosphorylates on the C-terminal serine cluster.which decreases activity and prevents prolonged activation of the inflammatory response. Phosphorylated by the IKK-related kinases TBK1 and IKBKE, which is associated with reduced CHUK/IKKA and IKBKB activity and NF-kappa-B-dependent gene transcription. Dephosphorylated at Ser-177 and Ser-181 by PPM1A and PPM1B. Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. 'Ser-163' may not serve as a monoubiquitination site. Ubiquitination on 'Ser-163' may modulate phosphorylation on C-terminal serine residues. Hydroxylated by PHD1/EGLN2, loss of hydroxylation under hypoxic conditions results in activation of NF-kappa-B.
Function Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NAA10, NCOA3, BCL10 and IRS1. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus.
Protein Name Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Beta
I-Kappa-B-Kinase Beta
Ikk-B
Ikk-Beta
Ikbkb
I-Kappa-B Kinase 2
Ikk2
Nuclear Factor Nf-Kappa-B Inhibitor Kinase Beta
Nfkbikb
Serine/Threonine Protein Kinase Ikbkb
Database Links Reactome: R-MMU-1169091
Reactome: -MMU-1810476
Reactome: -MMU-202424
Reactome: -MMU-209543
Reactome: -MMU-209560
Reactome: -MMU-2871837
Reactome: -MMU-445989
Reactome: -MMU-5357905
Reactome: -MMU-5357956
Reactome: -MMU-5607764
Reactome: -MMU-5684264
Reactome: -MMU-9020702
Reactome: -MMU-933542
Reactome: -MMU-937039
Reactome: -MMU-937041
Reactome: -MMU-975144
Reactome: -MMU-9758274
Cellular Localisation Cytoplasm
Nucleus
Membrane Raft
Colocalized With Dpp4 In Membrane Rafts
Alternative ELISA Names Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Beta ELISA kit
I-Kappa-B-Kinase Beta ELISA kit
Ikk-B ELISA kit
Ikk-Beta ELISA kit
Ikbkb ELISA kit
I-Kappa-B Kinase 2 ELISA kit
Ikk2 ELISA kit
Nuclear Factor Nf-Kappa-B Inhibitor Kinase Beta ELISA kit
Nfkbikb ELISA kit
Serine/Threonine Protein Kinase Ikbkb ELISA kit
Ikbkb ELISA kit
Ikkb ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance