| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This Hspa5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Hspa5 |
| Gene ID: |
14828 |
| Uniprot ID: |
BIP_MOUSE |
| Specificity: |
This kit recognizes Mouse GRP78 in samples. No significant cross-reactivity or interference between Mouse GRP78 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | In unstressed cells, AMPylation at Thr-519 by FICD inactivates the chaperome activity: AMPylated form is locked in a relatively inert state and only weakly stimulated by J domain-containing proteins. In response to endoplasmic reticulum stress, de-AMPylation by the same protein, FICD, restores the chaperone activity. |
| Function | Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1. Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation. |
| Protein Name | Endoplasmic Reticulum Chaperone Bip78 Kda Glucose-Regulated ProteinGrp-78Binding-Immunoglobulin ProteinBipHeat Shock Protein 70 Family Protein 5Hsp70 Family Protein 5Heat Shock Protein Family A Member 5Immunoglobulin Heavy Chain-Binding Protein |
| Database Links | Reactome: R-MMU-3371453Reactome: -MMU-983170 |
| Cellular Localisation | Endoplasmic Reticulum LumenMelanosomeCytoplasmCell SurfaceIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvLocalizes To The Cell Surface In Epithelial CellsHigh Levels Of Free Iron Promotes Cell Surface Localization |
| Alternative ELISA Names | Endoplasmic Reticulum Chaperone Bip ELISA kit78 Kda Glucose-Regulated Protein ELISA kitGrp-78 ELISA kitBinding-Immunoglobulin Protein ELISA kitBip ELISA kitHeat Shock Protein 70 Family Protein 5 ELISA kitHsp70 Family Protein 5 ELISA kitHeat Shock Protein Family A Member 5 ELISA kitImmunoglobulin Heavy Chain-Binding Protein ELISA kitHspa5 ELISA kitGrp78 ELISA kit |
| Specificity | This kit recognizes Mouse GRP78 in samples. No significant cross-reactivity or interference between Mouse GRP78 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-96 | 91 |
| EDTA plasma (n=8) | 92-107 | 98 |
| Cell culture media (n=8) | 93-110 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.94 | 2.59 | 6.83 | 1.01 | 2.65 | 6.27 |
| Standard deviation | 0.06 | 0.13 | 0.33 | 0.06 | 0.13 | 0.23 |
| CV (%) | 6.38 | 5.02 | 4.83 | 5.94 | 4.91 | 3.67 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
