• Mouse HO1 (Heme Oxygenase 1) CLIA Kit (STJC0001294)

Mouse HO1 (Heme Oxygenase 1) CLIA Kit (STJC0001294)

SKU:
STJC0001294

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Applications: CLIA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This mouse HO1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Hmox1
Gene ID: 15368
Uniprot ID: HMOX1_MOUSE
Specificity: This kit recognizes Mouse HO1 in samples. No significant cross-reactivity or interference between Mouse HO1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications A soluble form arises by proteolytic removal of the membrane anchor.
Function Heme oxygenase 1: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis. Heme oxygenase 1 soluble form: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
Protein Name Heme Oxygenase 1
Ho-1
P32 Protein Cleaved Into - Heme Oxygenase 1 Soluble Form
Database Links Reactome: R-MMU-189483
Reactome: -MMU-917937
Reactome: -MMU-9609523
Reactome: -MMU-9707564
Reactome: -MMU-9707587
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iv Membrane Protein
Cytoplasmic Side
Alternative CLIA Names Heme Oxygenase 1 CLIA kit
Ho-1 CLIA kit
P32 Protein Cleaved Into - Heme Oxygenase 1 Soluble Form CLIA kit
Hmox1 CLIA kit
Specificity This kit recognizes Mouse HO1 in samples. No significant cross-reactivity or interference between Mouse HO1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-105 96
EDTA plasma(n=8) 93-105 100
Cell culture media(n=8) 98-112 106

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 25.04 65.83 199.19 23.91 60.73 192.19
Standard deviation 3.25 4.85 17.03 3.04 4.78 16.05
CV (%) 12.98 7.37 8.55 12.71 7.87 8.35
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance