| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This mouse HNRPA1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Hnrnpa1 |
| Gene ID: |
15382 |
| Uniprot ID: |
ROA1_MOUSE |
| Specificity: |
This kit recognizes Mouse HNRPA1 in samples. No significant cross-reactivity or interference between Mouse HNRPA1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Sumoylated. |
| Function | Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection. Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform. Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1. May bind to specific miRNA hairpins. |
| Protein Name | Heterogeneous Nuclear Ribonucleoprotein A1Hnrnp A1Hdp-1Helix-Destabilizing ProteinSingle-Strand-Binding ProteinTopoisomerase-Inhibitor SuppressedHnrnp Core Protein A1 Cleaved Into - Heterogeneous Nuclear Ribonucleoprotein A1 - N-Terminally Processed |
| Database Links | Reactome: R-MMU-6803529Reactome: -MMU-72163Reactome: -MMU-72203 |
| Cellular Localisation | NucleusCytoplasmLocalized In Cytoplasmic Mrnp Granules Containing Untranslated MrnasShuttles Continuously Between The Nucleus And The Cytoplasm Along With MrnaComponent Of Ribonucleosomes |
| Alternative CLIA Names | Heterogeneous Nuclear Ribonucleoprotein A1 CLIA kitHnrnp A1 CLIA kitHdp-1 CLIA kitHelix-Destabilizing Protein CLIA kitSingle-Strand-Binding Protein CLIA kitTopoisomerase-Inhibitor Suppressed CLIA kitHnrnp Core Protein A1 Cleaved Into - Heterogeneous Nuclear Ribonucleoprotein A1 - N-Terminally Processed CLIA kitHnrnpa1 CLIA kitFli-2 CLIA kitHnrpa1 CLIA kitTis CLIA kit |
| Specificity | This kit recognizes Mouse HNRPA1 in samples. No significant cross-reactivity or interference between Mouse HNRPA1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-97 | 90 |
| EDTA plasma(n=8) | 86-102 | 93 |
| Cell culture media(n=8) | 95-109 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 51.21 | 133.18 | 443.87 | 52.57 | 122.83 | 401.74 |
| Standard deviation | 6.41 | 9.34 | 37.42 | 4.67 | 9.89 | 25.35 |
| CV (%) | 12.52 | 7.01 | 8.43 | 8.88 | 8.05 | 6.31 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
