| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This mouse HJV kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Hjv |
| Gene ID: |
69585 |
| Uniprot ID: |
RGMC_MOUSE |
| Specificity: |
This kit recognizes Mouse HJV in samples. No significant cross-reactivity or interference between Mouse HJV and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Muscle cell lineage. |
| Post Translational Modifications | Autocatalytically cleaved at low pH.the two chains remain linked via two disulfide bonds. Also proteolytically processed by TMPRSS6, several fragments being released in the extracellular space.regulates HJV activity in BMP signaling and thefore iron homeostasis. |
| Function | Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis. |
| Protein Name | HemojuvelinHemochromatosis Type 2 Protein HomologHemojuvelin Bmp CoreceptorRgm Domain Family Member C |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorAlso Released In The Extracellular Space |
| Alternative CLIA Names | Hemojuvelin CLIA kitHemochromatosis Type 2 Protein Homolog CLIA kitHemojuvelin Bmp Coreceptor CLIA kitRgm Domain Family Member C CLIA kitHjv CLIA kitHfe2 CLIA kitRgmc CLIA kit |
| Specificity | This kit recognizes Mouse HJV in samples. No significant cross-reactivity or interference between Mouse HJV and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-116 | 107 |
| EDTA plasma(n=8) | 92-105 | 100 |
| Cell culture media(n=8) | 101-116 | 108 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 211.83 | 579.49 | 1411.13 | 228.24 | 614.56 | 1299.24 |
| Standard deviation | 17.41 | 47.63 | 148.59 | 20.61 | 71.60 | 117.45 |
| CV (%) | 8.22 | 8.22 | 10.53 | 9.03 | 11.65 | 9.04 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
