| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.115ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This HADHb Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
Hadhb |
| Gene ID: |
231086 |
| Uniprot ID: |
ECHB_MOUSE |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Acetylation of Lys-202 is observed in liver mitochondria from fasted mice but not from fed mice. |
| Function | Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity. |
| Protein Name | Trifunctional Enzyme Subunit Beta - MitochondrialTp-Beta Includes - 3-Ketoacyl-Coa ThiolaseAcetyl-Coa AcyltransferaseBeta-Ketothiolase |
| Database Links | Reactome: R-MMU-1482798Reactome: -MMU-77285Reactome: -MMU-77305Reactome: -MMU-77310Reactome: -MMU-77346Reactome: -MMU-77348Reactome: -MMU-77350 |
| Cellular Localisation | MitochondrionMitochondrion Inner MembraneMitochondrion Outer MembraneEndoplasmic ReticulumProtein Stability And Association With Membranes Require Hadha |
| Alternative ELISA Names | Trifunctional Enzyme Subunit Beta - Mitochondrial ELISA kitTp-Beta Includes - 3-Ketoacyl-Coa Thiolase ELISA kitAcetyl-Coa Acyltransferase ELISA kitBeta-Ketothiolase ELISA kitHadhb ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
