| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.064ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This GbA Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
Gba1 |
| Gene ID: |
14466 |
| Uniprot ID: |
GBA1_MOUSE |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramides/GlcCers (such as beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine) into free ceramides (such as N-acylsphing-4-enine) and glucose. Plays a central role in the degradation of complex lipids and the turnover of cellular membranes. Through the production of ceramides, participates in the PKC-activated salvage pathway of ceramide formation. Catalyzes the glucosylation of cholesterol, through a transglucosylation reaction where glucose is transferred from GlcCer to cholesterol. GlcCer containing mono-unsaturated fatty acids (such as beta-D-glucosyl-N-(9Z-octadecenoyl)-sphing-4-enine) are preferred as glucose donors for cholesterol glucosylation when compared with GlcCer containing same chain length of saturated fatty acids (such as beta-D-glucosyl-N-octadecanoyl-sphing-4-enine). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl 3-beta-D-glucoside to ceramide. Can also hydrolyze cholesteryl 3-beta-D-glucoside producing glucose and cholesterol. Catalyzes the hydrolysis of galactosylceramides/GalCers (such as beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine), as well as the transfer of galactose between GalCers and cholesterol in vitro, but with lower activity than with GlcCers. Contrary to GlcCer and GalCer, xylosylceramide/XylCer (such as beta-D-xyosyl-(1<->1')-N-acylsphing-4-enine) is not a good substrate for hydrolysis, however it is a good xylose donor for transxylosylation activity to form cholesteryl 3-beta-D-xyloside. |
| Protein Name | Lysosomal Acid GlucosylceramidaseLysosomal Acid GcaseAcid Beta-GlucosidaseBeta-GlucocerebrosidaseCholesterol GlucosyltransferaseSgtaseCholesteryl-Beta-GlucosidaseD-Glucosyl-N-Acylsphingosine GlucohydrolaseLysosomal Cholesterol GlycosyltransferaseLysosomal GalactosylceramidaseLysosomal Glycosylceramidase |
| Database Links | Reactome: R-MMU-1660662 |
| Cellular Localisation | Lysosome MembranePeripheral Membrane ProteinLumenal SideInteraction With Saposin-C Promotes Membrane ?AssociationTargeting To Lysosomes Occurs Through An Alternative Mpr-Independent Mechanism Via Scarb2 |
| Alternative ELISA Names | Lysosomal Acid Glucosylceramidase ELISA kitLysosomal Acid Gcase ELISA kitAcid Beta-Glucosidase ELISA kitBeta-Glucocerebrosidase ELISA kitCholesterol Glucosyltransferase ELISA kitSgtase ELISA kitCholesteryl-Beta-Glucosidase ELISA kitD-Glucosyl-N-Acylsphingosine Glucohydrolase ELISA kitLysosomal Cholesterol Glycosyltransferase ELISA kitLysosomal Galactosylceramidase ELISA kitLysosomal Glycosylceramidase ELISA kitGba1 ELISA kitGba ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
