| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.115ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This FYN Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
Fyn |
| Gene ID: |
14360 |
| Uniprot ID: |
FYN_MOUSE |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Isoform 1 is highly expressed in the brain, isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes. |
| Post Translational Modifications | Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state. PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Ultraviolet B (UVB) strongly increase phosphorylation at Thr-15 and kinase activity, and promotes translocation from the cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling. Phosphorylated at tyrosine residues, which can be enhanced by NTN1. Palmitoylated. Palmitoylation at Cys-3 and Cys-6, probably by ZDHHC21, regulates subcellular location. Myristoylation is required prior to palmitoylation. |
| Function | Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling. |
| Protein Name | Tyrosine-Protein Kinase FynProto-Oncogene C-FynP59-Fyn |
| Database Links | Reactome: R-MMU-114604Reactome: -MMU-1227986Reactome: -MMU-1257604Reactome: -MMU-1433557Reactome: -MMU-1433559Reactome: -MMU-202733Reactome: -MMU-2029481Reactome: -MMU-210990Reactome: -MMU-2424491Reactome: -MMU-373753Reactome: -MMU-375165Reactome: -MMU-389356Reactome: -MMU-389357Reactome: -MMU-389359Reactome: -MMU-389513Reactome: -MMU-3928662Reactome: -MMU-3928663Reactome: -MMU-3928664Reactome: -MMU-3928665Reactome: -MMU-399954Reactome: -MMU-399955Reactome: -MMU-399956Reactome: -MMU-418885Reactome: -MMU-4420097Reactome: -MMU-5621480Reactome: -MMU-5621575Reactome: -MMU-5673001Reactome: -MMU-6811558Reactome: -MMU-75892Reactome: -MMU-8866376Reactome: -MMU-9032759Reactome: -MMU-912631 |
| Cellular Localisation | CytoplasmNucleusCell MembranePerikaryonPresent And Active In Lipid RaftsPalmitoylation Is Crucial For Proper Trafficking |
| Alternative ELISA Names | Tyrosine-Protein Kinase Fyn ELISA kitProto-Oncogene C-Fyn ELISA kitP59-Fyn ELISA kitFyn ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
