Name: Mouse Fndc5 (Fibronectin type III domain-containing protein 5) Sandwich ELISA Kit (STJE0004491)
SKU: STJE0004491
Availability: InStock

General Information
Product Properties
Target Information
Additional Info
Product Review

Applications:	ELISA
Reactivity:	Mouse
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	37.5pg/mL
Detection Limit:	62.5~4000pg/mL
Short Description :	This Fndc5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	Fndc5
Gene ID:	384061
Uniprot ID:	FNDC5_MOUSE
Specificity:	This kit recognizes Mouse FNDC5 in samples. No significant cross-reactivity or interference between Mouse FNDC5 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	The extracellular domain is cleaved and released from the cell membrane. N-Glycosylated.
Function	Irisin: Mediates beneficial effects of muscular exercise. Induces browning of white adipose tissue by stimulating UCP1 expression, at least in part, via the nuclear receptor PPARA.
Protein Name	Fibronectin Type Iii Domain-Containing Protein 5 Fibronectin Type Iii Repeat-Containing Protein 2 Peroxisomal Protein Pep Cleaved Into - Irisin
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Peroxisome Membrane Secreted Imported In Peroxisomes Through The Pex5 Receptor Pathway Irisin: Secreted Detected In The Blood Of Individuals Subjected To Endurance Exercise
Alternative ELISA Names	Fibronectin Type Iii Domain-Containing Protein 5 ELISA kit Fibronectin Type Iii Repeat-Containing Protein 2 ELISA kit Peroxisomal Protein ELISA kit Pep Cleaved Into - Irisin ELISA kit Fndc5 ELISA kit Frcp2 ELISA kit Pep ELISA kit
Specificity	This kit recognizes Mouse FNDC5 in samples. No significant cross-reactivity or interference between Mouse FNDC5 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-103	96
EDTA plasma (n=8)	93-104	98
Cell culture media (n=8)	88-99	93

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	194.11	575.25	1915.16	202.17	619.86	2032.21
Standard deviation	12.64	31.70	63.97	12.78	30.81	68.89
CV (%)	6.51	5.51	3.34	6.32	4.97	3.39

Model	Name	Reactivity	Application
STJE0019808	Human FNDC5 (Fibronectin type 3 domain-containing protein 5) Sandwich ELISA Kit (STJE0019808)	Human	ELISA
STJC0000998	Human FNDC5 (Fibronectin type III domain-containing protein 5) CLIA Kit (STJC0000998)	Human	CLIA
STJE0012283	Mouse SOSTDC1 (Sclerostin Domain Containing Protein 1) Sandwich ELISA Kit Kit (STJE0012283)	Mouse	ELISA

Mouse Fndc5 (Fibronectin type III domain-containing protein 5) Sandwich ELISA Kit (STJE0004491)

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