| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.94pg/mL |
| Detection Limit: |
1.56~100pg/mL |
| Short Description: |
This mouse FMOD kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Fmod |
| Gene ID: |
14264 |
| Uniprot ID: |
FMOD_MOUSE |
| Specificity: |
This kit recognizes Mouse FMOD in samples. No significant cross-reactivity or interference between Mouse FMOD and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Highest levels observed in knee epiphysis, in calvarial and diaphyseal bone, in nasal and costal cartilage, in the eye, and in bladder. In mature knee joint it is mostly present in the proliferating zone of growth plate. It is also observed in ligaments, especially at insertion sites, in the junction between meniscus and joint capsule, in the perimysium of skeletal muscle and in the periosteum. |
| Post Translational Modifications | Binds keratan sulfate chains. Sulfated on tyrosine residue(s). |
| Function | Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis. |
| Protein Name | FibromodulinFmCollagen-Binding 59 Kda ProteinKeratan Sulfate Proteoglycan FibromodulinKspg Fibromodulin |
| Database Links | Reactome: R-MMU-2022854Reactome: -MMU-2022857 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative CLIA Names | Fibromodulin CLIA kitFm CLIA kitCollagen-Binding 59 Kda Protein CLIA kitKeratan Sulfate Proteoglycan Fibromodulin CLIA kitKspg Fibromodulin CLIA kitFmod CLIA kit |
| Specificity | This kit recognizes Mouse FMOD in samples. No significant cross-reactivity or interference between Mouse FMOD and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 98 |
| EDTA plasma(n=8) | 95-110 | 101 |
| Cell culture media(n=8) | 86-99 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 4.67 | 11.53 | 37.31 | 5.07 | 11.72 | 38.47 |
| Standard deviation | 0.38 | 1.05 | 3.98 | 0.56 | 1.18 | 2.63 |
| CV (%) | 8.14 | 9.11 | 10.67 | 11.05 | 10.07 | 6.84 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
