| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.136ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This FLG Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
FlgUniprot ID=FILA_MOUSE" |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in the granular layer of the epidermis (at protein level). Expressed in the epidermis of the ear (at protein level). |
| Post Translational Modifications | Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 248 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. |
| Function | Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. |
| Protein Name | Filaggrin |
| Database Links | |
| Cellular Localisation | Cytoplasmic GranuleIn The Stratum Granulosum Of The EpidermisLocalized Within Keratohyalin GranulesIn Granular Keratinocytes And In Lower CorneocytesColocalizes With Calpain-1/Capn1 |
| Alternative ELISA Names | Filaggrin ELISA kitFlg ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
