• Mouse FASL (Factor Related Apoptosis Ligand) Sandwich ELISA Kit (STJE0008792)

Mouse FASL (Factor Related Apoptosis Ligand) Sandwich ELISA Kit (STJE0008792)

SKU:
STJE0008792

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Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.060ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This FASL Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: Faslg
Gene ID: 14103
Uniprot ID: TNFL6_MOUSE
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Tissue Specificity Expressed in T-cells. Expressed in natural killer cells.
Post Translational Modifications The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FAsL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells. Phosphorylated by FGR on tyrosine residues.this is required for ubiquitination and subsequent internalization. N-glycosylated. Glycosylation enhances apoptotic activity. Monoubiquitinated.
Function Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development. Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses. TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance. Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis. Tumor necrosis factor ligand superfamily member 6, soluble form: Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways. Can induce apoptosis but does not appear to be essential for this process. FasL intracellular domain: Cytoplasmic form induces gene transcription inhibition.
Protein Name Tumor Necrosis Factor Ligand Superfamily Member 6
Cd95 Ligand
Cd95-L
Fas Antigen Ligand
Fas Ligand
Fasl
Cd Antigen Cd178 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 6 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 6 - Soluble Form
Receptor-Binding Fasl Ectodomain
Soluble Fas Ligand
Sfasl - Adam10-Processed Fasl Form
Apl - Fasl Intracellular Domain
Fasl Icd
Sppl2a-Processed Fasl Form
Spa
Database Links Reactome: R-MMU-3371378
Reactome: -MMU-5218900
Reactome: -MMU-69416
Reactome: -MMU-75157
Cellular Localisation Isoform Fasl: Cell Membrane
Single-Pass Type Ii Membrane Protein
Cytoplasmic Vesicle Lumen
Lysosome Lumen
Is Internalized Into Multivesicular Bodies Of Secretory Lysosomes After Phosphorylation By Fgr And Monoubiquitination
Colocalizes With The Sppl2a Protease At The Cell Membrane
Tumor Necrosis Factor Ligand Superfamily Member 6
Soluble Form: Secreted
May Be Released Into The Extracellular Fluid By Cleavage From The Cell Surface
Fasl Intracellular Domain: Nucleus
The Fasl Icd Cytoplasmic Form Is Translocated Into The Nucleus
Alternative ELISA Names Tumor Necrosis Factor Ligand Superfamily Member 6 ELISA kit
Cd95 Ligand ELISA kit
Cd95-L ELISA kit
Fas Antigen Ligand ELISA kit
Fas Ligand ELISA kit
Fasl ELISA kit
Cd Antigen Cd178 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 6 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 6 - Soluble Form ELISA kit
Receptor-Binding Fasl Ectodomain ELISA kit
Soluble Fas Ligand ELISA kit
Sfasl - Adam10-Processed Fasl Form ELISA kit
Apl - Fasl Intracellular Domain ELISA kit
Fasl Icd ELISA kit
Sppl2a-Processed Fasl Form ELISA kit
Spa ELISA kit
Faslg ELISA kit
Apt1lg1 ELISA kit
Cd95l ELISA kit
Fasl ELISA kit
gld ELISA kit
Tnfsf6 ELISA kit
output

Information sourced from Uniprot.org

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