• Mouse Fabp2 (Fatty acid-binding protein, intestinal) Sandwich ELISA (STJE0004750)

Mouse Fabp2 (Fatty acid-binding protein, intestinal) Sandwich ELISA (STJE0004750)

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STJE0004750

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This Fabp2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Fabp2
Gene ID: 14079
Uniprot ID: FABPI_MOUSE
Specificity: This kit recognizes Mouse IFABP/FABP2 in samples. No significant cross-reactivity or interference between Mouse IFABP/FABP2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
Protein Name Fatty Acid-Binding Protein - Intestinal
Fatty Acid-Binding Protein 2
Intestinal-Type Fatty Acid-Binding Protein
I-Fabp
Database Links Reactome: R-MMU-163560
Cellular Localisation Cytoplasm
Alternative ELISA Names Fatty Acid-Binding Protein - Intestinal ELISA kit
Fatty Acid-Binding Protein 2 ELISA kit
Intestinal-Type Fatty Acid-Binding Protein ELISA kit
I-Fabp ELISA kit
Fabp2 ELISA kit
Fabpi ELISA kit
Specificity This kit recognizes Mouse IFABP/FABP2 in samples. No significant cross-reactivity or interference between Mouse IFABP/FABP2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-108 99
EDTA plasma (n=8) 88-101 96
Cell culture media (n=8) 89-103 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.49 1.36 4.80 0.53 1.28 4.48
Standard deviation 0.03 0.07 0.19 0.03 0.08 0.20
CV (%) 6.12 5.15 3.96 5.66 6.25 4.46
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance