| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This F8 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
F8 |
| Gene ID: |
14069 |
| Uniprot ID: |
FA8_MOUSE |
| Specificity: |
This kit recognizes Mouse F8 in samples. No significant cross-reactivity or interference between Mouse F8 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The binding of vWF and activation depend on the sulfation of Tyr-1669. Proteolytically cleaved by cathepsin CTSG to produce a partially activated form. |
| Function | Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. |
| Protein Name | Coagulation Factor ViiiProcoagulant Component |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-140837Reactome: -MMU-140875Reactome: -MMU-163841Reactome: -MMU-204005Reactome: -MMU-5694530 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative ELISA Names | Coagulation Factor Viii ELISA kitProcoagulant Component ELISA kitF8 ELISA kitCf8 ELISA kitF8c ELISA kit |
| Specificity | This kit recognizes Mouse F8 in samples. No significant cross-reactivity or interference between Mouse F8 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-109 | 102 |
| EDTA plasma (n=8) | 90-101 | 96 |
| Cell culture media (n=8) | 88-101 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.03 | 3.17 | 9.68 | 1.01 | 2.93 | 10.02 |
| Standard deviation | 0.05 | 0.15 | 0.48 | 0.06 | 0.17 | 0.53 |
| CV (%) | 4.85 | 4.73 | 4.96 | 5.94 | 5.80 | 5.29 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
