| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This mouse ER Beta kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Esr2 |
| Gene ID: |
13983 |
| Uniprot ID: |
ESR2_MOUSE |
| Specificity: |
This kit recognizes Mouse ER Beta in samples. No significant cross-reactivity or interference between Mouse ER Beta and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in prostate, ovary, Leydig cells and in epithelium of the efferent ductules and of the initial segment of the epididymis. |
| Post Translational Modifications | Phosphorylation at Ser-87 and Ser-105 recruits NCOA1. |
| Function | Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. |
| Protein Name | Estrogen Receptor BetaEr-BetaNuclear Receptor Subfamily 3 Group A Member 2 |
| Database Links | Reactome: R-MMU-1257604Reactome: -MMU-383280Reactome: -MMU-6811558Reactome: -MMU-8939211Reactome: -MMU-9009391 |
| Cellular Localisation | Nucleus |
| Alternative CLIA Names | Estrogen Receptor Beta CLIA kitEr-Beta CLIA kitNuclear Receptor Subfamily 3 Group A Member 2 CLIA kitEsr2 CLIA kitEstrb CLIA kitNr3a2 CLIA kit |
| Specificity | This kit recognizes Mouse ERβ in samples. No significant cross-reactivity or interference between Mouse ERβ and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-107 | 101 |
| EDTA plasma(n=8) | 98-110 | 104 |
| Cell culture media(n=8) | 102-116 | 108 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 193.44 | 348.18 | 1679.62 | 200.28 | 324.69 | 1649.17 |
| Standard deviation | 18.67 | 35.83 | 187.11 | 24.41 | 32.60 | 179.92 |
| CV (%) | 9.65 | 10.29 | 11.14 | 12.19 | 10.04 | 10.91 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
