| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.108ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This EGLN1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
Egln1 |
| Gene ID: |
112405 |
| Uniprot ID: |
EGLN1_MOUSE |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in heart, brain liver, skeletal muscle and kidney. Low levels were detected in the lung. Constitutively expressed during differentiation of C2C12 skeletal myocytes. |
| Post Translational Modifications | S-nitrosylation inhibits the enzyme activity up to 60% under aerobic conditions. Chelation of Fe(2+) has no effect on the S-nitrosylation. It is uncertain whether nitrosylation occurs on Cys-300 or Cys-303. |
| Function | Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif. |
| Protein Name | Egl Nine Homolog 1Hypoxia-Inducible Factor Prolyl Hydroxylase 2Hif-Ph2Hif-Prolyl Hydroxylase 2Hph-2Prolyl Hydroxylase Domain-Containing Protein 2Phd2Sm-20 |
| Database Links | Reactome: R-MMU-1234176 |
| Cellular Localisation | CytoplasmNucleusMainly CytoplasmicShuttles Between The Nucleus And CytoplasmNuclear Export Requires Functional Xpo1 |
| Alternative ELISA Names | Egl Nine Homolog 1 ELISA kitHypoxia-Inducible Factor Prolyl Hydroxylase 2 ELISA kitHif-Ph2 ELISA kitHif-Prolyl Hydroxylase 2 ELISA kitHph-2 ELISA kitProlyl Hydroxylase Domain-Containing Protein 2 ELISA kitPhd2 ELISA kitSm-20 ELISA kitEgln1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
