• Mouse DAG1 (Dystrophin Associated Glycoprotein 1) Sandwich ELISA Kit Ready-To-Use (STJE0014726)

Mouse DAG1 (Dystrophin Associated Glycoprotein 1) Sandwich ELISA Kit Ready-To-Use (STJE0014726)

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STJE0014726

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Applications: ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.054ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This DAG1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: Dag1
Gene ID: 13138
Uniprot ID: DAG1_MOUSE
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates or other biological fluids.
Tissue Specificity Detected in brain and kidney (at protein level). Detected in sciatic nerve (at protein level). Expressed in neurons and muscle cells (at protein level). Expressed in a variety of tissues. In brain, expressed in the hippocampal formation, the olfactory bulb, the cerebellum and the thalamus. In the peripheral nerve system, expressed in Schwann cells.
Post Translational Modifications Alpha-dystroglycan: O-glycosylated. POMGNT1 catalyzes the initial addition of N-acetylglucosamine, giving rise to the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety and thus providing the necessary basis for the addition of further carbohydrate moieties. Heavily O-glycosylated comprising of up to two thirds of its mass and the carbohydrate composition differs depending on tissue type. Mucin-type O-glycosylation is important for ligand binding activity. O-mannosylation is found in high abundance in both brain and muscle where the most abundant glycan is Sia-alpha-2-3-Gal-beta-1-4-Glc-NAc-beta-1-2-Man. In muscle, glycosylation on Thr-315, Thr-317, Thr-379 by a phosphorylated O-mannosyl glycan with the structure 2-(N-acetylamido)-2-deoxygalactosyl-beta-1,3-2-(N-acetylamido)-2-deoxyglucosyl-beta-1,4-6-phosphomannose is mediated by like-acetylglucosaminyltransferase (LARGE1) protein amd is required for laminin binding. O-glycosylated in the N-terminal region with a core 1 or possibly core 8 glycan. The brain form displays a unique glycosylation pattern which is absent in other tissues.this form shows enhanced binding to laminin LAMA5 compared to the skeletal muscle form. Beta-dystroglycan: N-glycosylated. Autolytic cleavage produces the alpha and beta subunits. In cutaneous cells, as well as in certain pathological conditions, shedding of beta-dystroglycan can occur releasing a peptide of about 30 kDa. SRC-mediated phosphorylation of the PPXY motif of the beta subunit recruits SH2 domain-containing proteins, but inhibits binding to WWW domain-containing proteins, DMD and UTRN. This phosphorylation also inhibits nuclear entry.
Function The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Alpha-dystroglycan: Extracellular peripheral glycoprotein that acts as a receptor for extracellular matrix proteins containing laminin-G domains, and for certain adenoviruses. Receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells. Also acts as a receptor for laminin LAMA5. Beta-dystroglycan: Transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity.
Protein Name Dystroglycan 1
Dystroglycan
Dystrophin-Associated Glycoprotein 1 Cleaved Into - Alpha-Dystroglycan
Alpha-Dg - Beta-Dystroglycan
Beta-Dg
Database Links Reactome: R-MMU-5173105
Reactome: -MMU-9010553
Cellular Localisation Alpha-Dystroglycan: Secreted
Extracellular Space
Beta-Dystroglycan: Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasm
Cytoskeleton
Nucleus
Nucleoplasm
Cell Membrane
Sarcolemma
Postsynaptic Cell Membrane
The Monomeric Form Translocates To The Nucleus Via The Action Of Importins And Depends On Ran
Nuclear Transport Is Inhibited By Tyr-892 Phosphorylation
In Skeletal Muscle
This Phosphorylated Form Locates To A Vesicular Internal Membrane Compartment
In Muscle Cells
Sarcolemma Localization Requires The Presence Of Ank2
While Localization To Costameres Requires The Presence Of Ank3
Localizes To Neuromuscular Junctions (Nmjs)
In Adult Muscle
Nmj Localization Depends Upon Ank2 Presence
But Not In Newborn Animals
In Peripheral Nerves
Localizes To The Schwann Cell Membrane
Colocalizes With Erm Proteins In Schwann-Cell Microvilli
Alternative ELISA Names Dystroglycan 1 ELISA kit
Dystroglycan ELISA kit
Dystrophin-Associated Glycoprotein 1 Cleaved Into - Alpha-Dystroglycan ELISA kit
Alpha-Dg - Beta-Dystroglycan ELISA kit
Beta-Dg ELISA kit
Dag1 ELISA kit
output

Information sourced from Uniprot.org

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